Hormonal checks are performed by taking blood in order to detect hormones that may affect male reproductive function, and which, in some cases, may cause subfertility. Hormones that are normally checked are FSH, LH, testosterone, prolactin, as well as thyroid hormones, because they are considered to possibly affect the reproductive system function. The most important of these hormones if FSH, which, if found at high levels, is an indicator that there may be a problem in spermatozoic production.
Mandatory tests performed in accordance to Law 3305/05 are carried out by taking a blood sample and checking for antibodies against Hepatitis A, B, C, HIV (I, II) and syphilis, before any attempted at assisted reproduction is made.